enogene/Rb多克隆抗体/100ul/E90003
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产品分类：
抗体套装
公司分类：
Antibody_set
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Rb Polyclonal AntibodyPDF
Catalog Number:
E90003Amount:
100ulBackground:
The retinoblastoma tumor suppressor protein, Rb, regulates cell proliferation by controlling progression through the restriction point within the G1-phase of the cell cycle (1). Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase, and proteins with a conserved LXCXE motif (2-4). Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression (5). Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation (6). Specificity of different CDK/cyclin complexes has been observed in vitro (6-8) and cyclin D1 is required for Ser780 phosphorylation in vivo (9).Species:
RabbitIsotype:
IgGStorage/Stability:
Store at -20oC or -80oC. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Altername:
retinoblastoma 1; RB1; OSRC; RB; p105-Rb; pRb; pp110Immunogen:
Fusion protein of human RbPurification:
Affinity purificationReactivity:
H MApplications:
WB IHCMolecular Weight:
110kDaSwiss-Prot No.:
P06400Gene ID:
5925References:
1.Sherr, C.J. (1996) Science 274, 1672-7. 2.Nevins, J.R. (1992) Science 258, 424-9. 3.Welch, P.J. and Wang, J.Y. (1993) Cell 75, 779-90. 4.Hu, Q.J. et al. (1990) EMBO J 9, 1147-55. 5.Knudsen, E.S. and Wang, J.Y. (1997) Mol Cell Biol 17, 5771-83. 6.Lundberg, A.S. and Weinberg, R.A. (1998) Mol Cell Biol 18, 753-61. 7.Connell-Crowley, L. et al. (1997) Mol Biol Cell 8, 287-301. 8.Kitagawa, M. et al. (1996) EMBO J 15, 7060-9. 9.Geng, Y. et al. (2001) Proc Natl Acad Sci USA 98, 194-9.WB 1:500 - 1:1000IHC 1:50 - 1:100
Western blot analysis of Jurkat cell and A431cell using Rb antibody.
品牌介绍
enogene的β-actin小鼠单克隆抗体（A02）PDF目录编号：E12-045量：100微克/ 100微升克隆编号：A02背景：β-肌动蛋白是已鉴定出的六种不同的肌动蛋白同工型之一。在各种物种和组织的细胞中发现的肌动蛋白分子在免疫学和物理特性方面趋于非常相似。因此，针对β-肌动蛋白的抗体可用作蛋白质印迹的上样对照。但是，应注意，β-肌动蛋白的水平在某些细胞中可能不稳定。例如，β-肌动蛋白在脂肪组织中的表达非常低，因此不应将β-肌动蛋白用作这些组织的负荷控制。抗体形式：pH 7.4、150mM NaCl，0.02％叠氮化钠和50％甘油的磷酸盐缓冲盐水（不含Mg2 +和Ca2 +）中的小鼠IgG1。储存/稳定性：-20℃/ 1年保存特异性/敏感性：抗体可以检测内源性β-肌动蛋白。反应性：H，R，M，Mk，Dg，Ch，Hm，Rb，Pg，Sh应用范围：WB：1：5,000-10,000 IHC：1：200 IF：1：200
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